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動(dòng)物行為軌跡分析平臺(tái)

來源:安微正華生物儀器設(shè)備有限公司   2014年12月09日 03:24  

6. 黑白箱實(shí)驗(yàn)(light dark box)

  The subject is placed in the dark portion of the box for a set period of acclimation time.  At the end of this period, a door separating the two compartments is opened.  The amount of time that the subject takes to emerge fully from the enclosed area into the open area is measured.

 

 

7. 社交行為實(shí)驗(yàn)(social interaction)

  The subject is habituated to the test chamber and allowed to freely explore for a set time.   A novel animal is placed in one of the two enclosures, and the percentage of time the mouse spends in the section with the novel animal is compared to the time spent in the section with the empty enclosure.   In a later session, the time spent with the same animal might be compared to time spent with a newer, more novel animal.

 

 

8. 條件性位置偏愛(Conditioned Place Preference)

   條件性位置偏愛實(shí)驗(yàn)(Conditioned Place Preference, CPP)實(shí)驗(yàn)是目前評(píng)價(jià)藥物精神依賴性的經(jīng)典實(shí)驗(yàn)?zāi)P停彩菑V泛應(yīng)用于尋找抗覓藥行為的有效工具。該實(shí)驗(yàn)將實(shí)驗(yàn)動(dòng)物(大鼠、小鼠)置于條件性位置偏愛箱的白色觀察區(qū),并給予精神依賴性藥物(例如*),然后觀察實(shí)驗(yàn)動(dòng)物在條件性位置偏愛箱的黑色區(qū)和白色區(qū)的活動(dòng)情況,白色區(qū)、黑色區(qū)以及其中的灰色 區(qū)之間有小門可供動(dòng)物自由穿梭。動(dòng)物每次處于給藥區(qū)就會(huì)在藥物獎(jiǎng)賞性效應(yīng)的作用下對(duì)黑色和白色區(qū)域產(chǎn)生位置上的偏好,其程度與藥物的精神依賴性相關(guān)。

9.   懸尾

懸尾實(shí)驗(yàn)主要用于抗抑郁、鎮(zhèn)靜以及止痛類藥物的研究。懸尾實(shí)驗(yàn)系統(tǒng)適用于大鼠、小鼠或其他實(shí)驗(yàn)室動(dòng)物,通過固定動(dòng)物尾部使其頭向下懸掛,記錄處于該環(huán)境的動(dòng)物產(chǎn)生絕望的不動(dòng)狀態(tài)過程中的一系列參數(shù)。

10. 條件性恐懼實(shí)驗(yàn)(fear conditioning)

條件性恐懼分析用于小型嚙齒類動(dòng)物(大、小鼠)環(huán)境相關(guān)條件性恐懼實(shí)驗(yàn)研究。抗抑郁藥和抗中樞興奮藥可以明顯縮短不動(dòng)狀態(tài)持續(xù)的時(shí)間。實(shí)驗(yàn)過程中,實(shí)驗(yàn)對(duì)象被給與一個(gè)聲音信號(hào),隨后給予電擊刺激。該訓(xùn)練稱為條件性訓(xùn)練,訓(xùn)練結(jié)束后實(shí)驗(yàn)動(dòng)物進(jìn)行聲音信號(hào)或環(huán)境性實(shí)驗(yàn)。一般情況下嚙齒類動(dòng)物對(duì)相應(yīng)的環(huán)境和不同環(huán)境下同樣的聲音信號(hào)都會(huì)做出明顯的條件性恐懼反應(yīng),如靜止不動(dòng)。

11. 震驚條件反射(startle and pre-pulse inhibition)

   ZH-ZJT型震驚反射系統(tǒng)用于研究動(dòng)物對(duì)突然的強(qiáng)烈刺激的反應(yīng)以及數(shù)據(jù)分析,一臺(tái)電腦可以控制1~5個(gè)震驚反應(yīng)箱,噪聲刺激可同時(shí)加入光、電、氣體進(jìn)行刺激,根據(jù)用戶自定義間隔給出相應(yīng)刺激,同時(shí)為恐懼誘導(dǎo)性驚愕反應(yīng)時(shí)間、潛伏應(yīng)答、震驚幅度,將在結(jié)果中顯示和存儲(chǔ)以便進(jìn)一步統(tǒng)計(jì)平價(jià)分析。數(shù)據(jù)均可導(dǎo)入ExcelSPSS統(tǒng)計(jì)方便。

Pre-Pulse Inhibition

In Pre-Pulse Inhibition (PPI), the startle stimulus is paired with a predictive cue. In normal subjects, the "pre-pulse" cue reduces the startle amplitude.  This inhibition of the startle response is known as PPI.  Humans and animal models of several disease states are known to have pre-pulse inhibition deficits, including schizophrenia, Alzheimer’s, and PTSD.

Fear-Potentiated Startle

In Fear-Potentiated Startle, the subjects are trained to associate a neutral stimulus, such as a light cue, with an aversive stimulus.  When the startle response is tested in the presence of the light cue, the startle amplitude should be potentiated, or increased.

      12.新穎物體識(shí)別(novel object recognition)

      The subject is habituated to the test chamber and allowed to freely explore for a set time.   A novel object is placed in one of the two enclosures, and the percentage of time the mouse spends in the section with the new object is compared to the time spent in the section with the empty enclosure.   In a later session, the time spent with the same object might be compared to time spent with a newer, more novel object.

 

 

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