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90Plus 納米粒度儀應用案例-3
點擊次數：230 發布時間：2014-12-7
文獻名：Formulation optimization for the nanoparticles-in-microsphere hybrid oral delivery system using factorial design
作者： Mayank D. Bhavsar, Sandip B. Tiwari, Mansoor M. Amiji
Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University, Boston, MA 02115, United States
摘要：The tremendous progress witnessed in the field of biotechnology with respect to discovery of therapeutic and antigenic proteins has propelled the need for development of suitable oral delivery devices for these and other macromolecules. In this study, we report the encapsulation of fluorescein isothiocyanate (FITC)-labeled gelatin nanoparticles into poly(ε-caprolactone) (PCL) microsphere (nanoparticle-in-microsphere oral delivery system, NiMOS) by double emulsion like technique and the influence of variables such as polymer concentration in organic phase, amount of nanoparticles added as internal phase, and the speed of homogenization on particle size of NiMOS using a 33 randomized full factorial design. A statistical model with interaction terms was derived to predict the particle size of the hybrid system. The results from multiple linear regression analysis and Student's t-test revealed that for obtaining large particles of NiMOS, a high polymer concentration and low speed of homogenization was necessary. In contrast, to obtain particles of smaller size, high speed of homogenization was found to be very important. The mathematical model obtained was validated for prediction of particle size. The encapsulation of gelatin nanoparticles in PCL microsphere was confirmed by fluorescent microscopy. Based on the statistical model we were also successful in producing NiMOS of less than 10 μm in size, which could be used as oral delivery system for therapeutic and antigenic macromolecules.
關鍵詞：Nanoparticles-in-microsphere hybrid delivery system; Oral delivery; Factorial design; Response surface plot